谷物及饲料中青霉酸的污染和防控.DOC

1、谷物及饲料中青霉酸的污染和防控李晓雪 1,2 董燕婕 1 苑学霞 1 丁照华 3 李大鹏 2 赵善仓 1,（山东省农业科学院农业质量标准与检测技术研究所/山东省食品质量与安全检测技术重点实验室 1，济南 250100）（山东农业大学食品科学与工程学院 2，泰安 271018）（山东省农业科学院玉米研究所 3，济南 250100）摘 要 青霉酸是由不同株系的青霉菌和曲霉菌产生的次生代谢产物，具有致突变和致癌作用。圆弧青霉和软毛青霉是主要产青霉酸真菌，主要存在于谷类和饲料中。青霉酸的生物合成途径约由 6 步构成，1 分子乙酰辅酶 A 同 3 分子丙二酸单酰辅酶 A 经缩合反应生成苔色酸，苔色酸经环

2、裂解和脱羧作用而生成青霉酸。青霉酸具有细胞毒性、器官毒性、混合毒性。控制谷物和饲料原料质量、贮藏及其加工运输过程，特别是控制好饲料水分和温度是控制青霉酸污染的关键。传统的检测方法包括生物学方法、免疫学方法、色谱法、质谱法等，目前单一种类真菌毒素的检测技术逐渐被多组分同时检测的方法代替。关键词 谷物 饲料 青霉酸 生物合成 毒性活性 检测 风险防控中图分类号：TS201.6 文献标志码： A 文章编号：1003-0174（2018） - -12The contamination and control of penicillic acid in cereals and feedsLi Xiao-

3、xue1,2 Dong Yan-jie1 Yuan Xue-xia1 Ding Zhao-hua3 Li Da-peng2Zhao Shan-cang1,(Institute of Agricultural Standards and Testing Technology for Agri-Products；Shandong Academy of Agricultural Sciences biochemical and toxicological investigations of Penicillium puberulum and Penicillium sloloniferumJ. Th

4、e United States Department of Agriculture, Bureau of Plant Industry,1913,270:147. 6 THORPE C W, JOHNSON R L. Analysis of penicillic acid by gas-liquid chromatographyJ. Journal Association of Official Analytical Chemists,1974,57(4):861-8657 CASQUETE R, RODRIGUEZ A, HERNANDEZ A, et al. Occurrence of T

5、oxigenic Fungi and Mycotoxins during Smoked Paprika ProductionJ. Journal of Food Protection,2017,80(12):2068-20778 HALLAS-MOLLER M, NIELSEN K F, FRISVAD J C. Production of the Fusarium Mycotoxin Moniliformin by Penicillium melanoconidiumJ. Journal of Agricultural and Food Chemistry,2016,64(22):4505-

6、45109 陈智, 邬静, 袁慧. 青霉酸研究进展J. 中国畜牧兽医,2007,34 (6): 28-29CHEN Z, WU J, YUAN H. Research process of penicillic acidJ. Chinese Animal Husbandry and Veterinarian,2007,34 (6): 28-2910SEKIGUCHI J, YAMADA Y, et al. 6-Methyl-1,2,4-benzenetriol,a new intermediate in penicillic acid biosynthesis in Penicillium c

7、yclopiumJ. Applied and Environmental Microbiology,1987,53(7): 1531-153511BENTLEY R, KEIL J G. Tetronic acid biosynthesis in moulds. II. Formation of penicillic acid in Penicillium cyclopium J. Journal of Biological Chemistry,1962,237:86787312AXBERG K , CATENBECK S. The enzymic formation of penicilli

8、c acidJ. FEBS Letters,1975, 54(1):18-2013AXBERG K , CATENBECK S. Intermediates in the penicillic acid biosynthesis in Penicillium cyclopiumJ. Acta Chem Scand B,1987,53(7):1531153514BACHA N. Caracterization of the polyketide synthases involved in biosynthesis of ochratoxin A, penicillic acid, asperla

9、ctone and isoasperlactone in aspergillus westerdijkiae (a molecular approach) D Toulouse. Institut National Polytechnique De Toulouse,200915SORENSON W G, SIMPSON J. Toxicity of penicillic acid for rat alveolar macrophages in vitroJ. Environmental Research,1986,41(2):505-51316GRABSCH C, WICHMANN G, L

10、OFFHAGEN N, et al. Cytotoxicity assessment of gliotoxin and penicillic acid in Tetrahymena pyriformisJ. Environmental Toxicology,2006,21(2):111-11717EZZAT S M, EI-SAYED E A, EI-HAWA A, et al. Morphological and ultrastructural studies for the biological action of penicillic acid on some bacterial spe

11、ciesJ. Research Journal of Microbiology,2007(2):303-31418KOMAGATA D, FUJITA S, YAMASHITA N, et al. Novel neuritogenic activities of pseurotin A and penicillic acidJ. The Journal of Antibiots,1996,49(9): 958-95919CIEGLER A, MINTZLAFF H J, WEISLEDER D, et al. Potential production, and detoxification o

12、f penicillic acid in mold-fermented sausage (salami)J. Applied Microbiology,1972,24(1): 114-11920袁慧,李文平.家兔圆弧青霉毒素中毒的临床与病理学观察J. 湖南农业大学学报,1999,25(3):233235YUAN H, LI W P. The clinical and therapeutic observation of cyclopenin in rabbitsJ. Hunan Agriculture University Newspaper, 1999,25(3): 233-23521PAZ

13、HANIVEL N, BALACHANDRAN C, MANOHA B M, et al. Effect of sublethaldose of Penicillic Acid toxicity on Growth, immunity, and lymphoid organs in Broiler ChickenJ. International Journal of Food, Agriculture and Veterinary Sciences,2014,4(3):105-11022HAYES A W, UNDER P D, WILLIAMS W L. Acute toxicity of

14、penicillic acid and rubratoxin B in dogsJ. Ann Nutr Aliment, 1977, 31(4-6): 711-72123STOEV S D，S TEGANOV M, DENEV S, et al. Experiment mycotoxincosis Chichens induced by Ochratoxin A and intervention with natural plant extractsJ. Veterinary Research Communications,2004,28(8): 727-74624SUZUKI S, KIMU

15、RA T, SAITO F, et al. Antitumor and antiviral properties of penicillic acidJ. Journal of the Agricultural Chemical Society,1971,35(2): 28729025KIMMEL J L, TIPTON P A. Inactivation of GDP-mannose dehydrogenase from Pseudomonas aeruginosa by penicillic acid identifies a critical active site loopJ. Arc

16、hives of Biochemistry and Biophysics,2005,441(2):132-14026OH S Y, CEDERGREEN N, YIANNIKOURIS A, et al. Assessing interactions of binary mixtures of Penicillium mycotoxins (PMs) by using a bovine macrophage cell line (BoMacs)J. Toxicology & Applied Pharmacology,2017,318:33-4027STOEV S D, DUTTON M, NJ

17、OBEH P, et al. Mycotoxic nephropathy in Bulgarian pigs and chickens: Complex aetiology and similarity to Balkan enedemic nephropathyJ. Food Additives & Contaminants Part A,2010,27(1):72-8828STOEV S D, GOUNDASHEVA D, MIRTCHEVA T, et al. Susceptibility to secondary bacterial infections in growing pigs

18、 as an early response in ochratoxicosisJ. Experimental & Toxicologic Pathology,2000,52(4):287-9629OH S, BALCH C G, CLIFF RL, et al. Exposure to Penicillium mycotoxins alters gene expression of enzymes involved in the epigenetic regulation of bovine macrophages(BoMacs)J. Mycotoxin Research,2013,29(4)

19、: 235-243.30REISS J. Inhibitory action of the mycotoxine, patulin and penicillic acid on ureaseJ. Food and Cosmetics Toxicology,1979,17(2):145-146.31SORENSON W G, SIMPSON J. Toxicity of penicillic acid for rat alveolar macrophages in vitroJ. Environmental Research,1986,41(2):505-51332PHILLIPS T D, C

20、HAN P K, HAYES A W. Inhibitory characteristics of the mycotoxin penicillic acid on (Na+-K+)-activated adenosine triphosphatesJ. Biochemical Pharmacology,1980, 29(1):19-2633BANDO M,HASEGAWA M, TSUBOI, et al. The mycotoxin penicillic acid inhibits Fas ligand-induced apoptosis by blocking self-processi

21、ng of caspase-8 in death-inducing signaling complexJ. Journal of Biological Chemistry,2003,278(8): 5786-579334QIN X, SUN Q, YANG B, et al. Quorum sensing influences phage infection efficiency via affecting cell population and physiological state J.Journal of Basic Microbiology,2016,57 (2):162-17035L

22、EI H Y, HE Z P, YUAN H. Generation, and characterization of a monoclonal antibody to penicillic acid from Penicillium cyclopiumJ. African Journal of Biotechnology,2010, 9(21):3026-303136郭成志,朱丽,鲁银,等.圆弧青霉菌毒素- 青霉酸间接竞争 ELISA 检测方法的建立J. Animal Husbandry & Veterinary Medicine, 2010,42(8):52-54GUO Z C, ZHU

23、L, LU Y, et al. Arcs penicillium penicillic acid indirect competition ELISA detection method was establishedJ. Animal Husbandry & Veterinary Medicine, 2010,42(8): 52-5437朱丽,雷红宇,袁慧. 胶体金标记抗圆弧青霉菌毒素-青霉酸单克隆抗体的研究 J. 中国畜牧兽医,2010,37(8): 30-33ZHU L, LEI H Y, YUAN H. The study of monoclonal antibody against cycle-penicillium in colloidal goldJ. Chinese Animal Husbandry and Veterinarian, 2010,37(8): 30-3338GOLINSKI P, GRABARKIEWICZ-SZCZESNA J. Chemical confirmatory tests for ochratoxin A, citrinin, penicillic acid, sterigmatocystin, and zearalenone performed directly on