06-Leukocyte-differentiation-antigen.pptx

1、Leukocyte differentiation antigenTTCRCD3BBCRTypes of cell communication 1. Direct-adhesion and interaction2. Indirect-secrete cytokinesThe basis for cell communication :cell surface molecule/markerfunction featureQuestion:How to distinguish T and B cells?l The cell surface markers which may appear o

2、n or disappear from cell membrane when stem cell differentiate into different cell lines or when blood cells are in differentiation stages and activation status.Leukocyte differentiation antigen(LDA): TTCRCD3BBCRImportantLDAn Expressed on leukocyte, erythrocyte and megakaryocytes or thrombocytesn Ot

3、her non-hematopoietic cellsn Trans-membrane protein or glycoproteinn Some are anchored on plasma membrane with GPI conjunction CD (cluster of differentiation) : Cell surface molecules of leukocytes that are distinguishable with monoclonal antibodies as an immunologic marker.Important分 组 CD分子（举例）T 细胞

4、 CD2、 CD3、 CD4、 CD5、 CD8、 CD28、 CD152（ CTLA4）、 CD154（ CD40L）B 细胞 CD19、 CD20、 CD21、 CD40、 CD79a（ Ig）、 CD79b（ Ig）、 CD80（ B7-1）、 CD86（ B7-2）髓样细胞 CD14、 CD35（ CR1）、 CD64（ FcR ）血小板 CD36、 CD41CD42aCD42d、 CD51、 CD61、 CD62P（ P 选择素）NK 细胞 CD16（ FcR ）、 CD56（ NCAM-1）、 CD94、 CD158（ KIR）、 CD161（ NKR-P1A）非谱系 CD30、

5、CD32（ FcR ）、 CD45RA、 CD45RO、 CD46（ MCP）、 CD55（ DAF）、 CD59黏附分子 CD11aCD11c、 CD15、 CD15s（ sLex）、 CD18（整合素 2）、 CD29（整合素 1）、 CD49aCD49f、CD54（ ICAM-1）、 CD62E（ E 选择素）、 CD62L（ L 选择素）细胞因子 /趋化性细胞因子受体CD25（ IL-2R）、 CD95（ Fas）、 CD116CDw137、 CD178（ FasL）、 CD183（ CXCR3）、 CD184（ CXCR4）、 CD195（ CCR5）内皮细胞 CD105（ TGF-

6、R ）、 CD106（ VCAM-1）、 CD140（ PDGFR）、 CD144（ VE 钙黏素）碳水化合物结构 CD15u、 CD60aCD60c、 CD75树突状细胞 CD83， CD80， CD86， CD85（ ILT/LIR）干细胞 /祖细胞 CD133、 CD243造血干细胞 CD34， CD117红细胞 CD233CD242Mature TDouble-positiveDouble-negativePre-TStem cellCD7 CD2 CD2 CD2CD3 CD3 CD3 CD4 CD4+CD8 or CD8CD4CD8ExampleBone marrow ThymusM

7、agnetic activated cell sorting (MACS)separate cells from complex mixtures using antibody-coated magnetic nanoparticles. The antibodies are specific for certain cell surface markers, either expressed on your population of interest (positive selection), or expressed on undesired cell types (negative s

8、election).Fluorescence-activated cell sorting (FACS) It is a specialized type of flow cytometry. the initial complex cell mixture is first labeled with one or more cell surface marker-specific antibodies that have been fused to fluorescent dyes. A vibrating mechanism causes the stream of cells to br

9、eak into individual droplets. Just before the stream breaks into droplets, the flow passes through a fluorescence measuring station where the fluorescent character of each cell of interest is measured. An electrical charging ring is placed just at the point where the stream breaks into droplets. The charged droplets then fall through an electrostatic deflection system that diverts droplets into containers based upon their charge.