1、,北京地区口腔综合治疗台用水的污染现状与控制方法探讨,2015/9/9,Su Jing D.D.S,1,苏,静,Dr. SU Jing, DDS首都医科大学附属北京口腔医院北京市口腔医疗质量控制和改进中心Beijing Stomatological Hospital, Capital Medical University,牙科用水标准,Water Standard In Dental Settings, US CDC2003年规定牙科综合治疗台用水应满足饮用水标准,500 CFU/ml US ADA建议参照透析用水,200 CFU/ml CHINA 市政水细菌含量的要求,100CFU/ml细菌
2、培养方法有差异Cultivation Difference,2015/9/9,2,Su Jing D.D.S,2015/9/9,3,Su Jing D.D.S,Poor Water QualityCause?浮游生物出水端水质超标生物膜脱落,2015/9/9,Su Jing D.D.S,4,水静止,水有菌生物膜形成5天后水路内水的细菌含量可达100,000CFU/ml,2015/9/9,5,Su Jing D.D.SBiofilm formation,浮游生物生物膜脱落,出水端水质超标,供水质量污水回吸,2015/9/9,6,Su Jing D.D.S,Poor Water QualityCa
3、use?,市政水,无菌水(罐),?,2015/9/9,7,Su Jing D.D.S,Input water from different ways,potable quality mains water,reservoir bottles中心水处理供水,water storage tanks,供水质量supply water quality,2015/9/9,8,Su Jing D.D.S,牙科综合治疗台出水端水质监测DCU Output Water Quality2012-2013感染可能免疫低下人群组织愈合受影响内毒素,三级口腔专科:1家*6台*3时点二级口腔专科: 3家 * 3台*3时
4、点三级综合医院口腔科:5家 * 3台*3时点二级综合医院口腔科:9家 * 3台*3时点专科门诊部、诊所: 18家 * 3台*3时点Dental units: 111Total sample: 327,采样对象,Sampling object,Sample name北京口腔医院北京友谊医院,北京中医药大学东方医院北京康复研究中心博爱医院北京协和医院北京安贞医院北京大兴区医院北京市石景山医院北京市第六医院北京市第二医院北京市日新口腔诊所北京市崇文区口腔医院北京菲格口腔门诊部北京奥德口腔诊所北京瑞可口腔门诊部北京东区口腔医院,2015/9/9,Su Jing D.D.S,9,采样 Sampling1
5、、Sampling:output water from dental handpiece, 10ml in sterilized tube酒精棉擦拭牙科手机管线出水口,干燥后,喷水,无菌试管采水样10ml,标编号;2、Control Sample: tap water or water in reservoir无菌试管采市政水样或者桶装水(根据医院的实际使用情况而定)10ml;3、Time Points:8AM、11AM、3PM4、Send Sample:100,0,0-100 100,0,0-100 100,0,0-100 100,8:0012:00,99,11,00,1010,00,00,
6、89,11,10,153160,169,11,90%,10%,0,100%,0,0,85%,10%,5%,92% 7.4% 0.6%,合格率,100%,100%,95%,99.4%,2015/9/9,Su Jing D.D.S,29,微酸性次氯酸水应用实验1、有效氯含量31-46mg/L;PH=5.4-7.35,2015/9/9,Su Jing D.D.S,30,微酸性次氯酸水应用实验2、次氯酸浓度15-25mg/L;PH=5.18-7.6,1d,N=20,2d N=20,3d,N=20,4-10d,N=140,0,0-100 100,0,0-100 100,0,0-100 100,0,0-1
7、00 100,8:0012:00,88,20,02,88,21,01,99,11,00,5968,102,10,80%,10%,10%,80% 15%,5%,90% 10%,0%,90.7%,8.6% 0.7%,合格率,90%,95%,100%,99.3%,2015/9/9,31,Su Jing D.D.S微酸性次氯酸水应用实验3、停用微酸性次氯酸水7天连续应用7天蒸馏水的水质合格率,1-7d,N=140,1d,2d,3d,4d,5d,6d,7d,合格100cfu/ml合格率,200100%,81240%,51525%,41620%,21810%,21810%,0200%,2015/9/9,S
8、u Jing D.D.S,32,微酸性次氯酸水应用实验4、有效氯含量7-10mg/L;PH=7.25-7.55,1d,N=20,2d N=20,3d,N=20,4-10d,N=140,0,0-100 100,0,0-100 100,0,0-100 100,0,0-100,100,8:0012:00,88,20,02,88,21,01,99,11,00,5968,102,10,80%,10%,10%,80% 15%,5%,90% 10%,0%,90.7% 8.6%,0.7%,合格率,90%,95%,100%,99.3 %,2015/9/9,Su Jing D.D.S,33,有效氯含量(mg/L)
9、合格,不合格,合计,有效率(%),31-4615-257-10合计,397196373966,34714,400200380980,99.2598.0098.1698.57,2=2.23,P0.1,微酸性次氯酸水三种浓度消毒效果比较2=2.23,P0.1微酸性次氯酸水应用实验5、微酸性次氯酸水三种浓度消毒效果比较,2015/9/9,Su Jing D.D.S,34,微酸性次氯酸水应用实验不同时间水质合格率的比较不同采样点水质合格率的比较无显著差异,生物膜控制抑制生成,清洗消毒一次,35Su J生物膜定期清除每周至少消毒剂,持续抑菌水,厚德博才,严精勤谨,38,Su Jing D.D.S,2015/9/9,感谢聆听!苏 静首都医科大学附属北京口腔医院北京市口腔医疗质量控制和改进中心,