犬血清淀粉样蛋白A(SAA)酶联免疫分析（ELISA）.DOC

1、1犬血清淀粉样蛋白 A（SAA）酶联免疫分析（ELISA）试剂盒使用说明书本试剂仅供研究使用 目的：本试剂盒用于测定犬血清，血浆及相关液体样本中血清淀粉样蛋白 A（SAA）的含量。实验原理：本试剂盒应用双抗体夹心法测定标本中犬血清淀粉样蛋白 A（SAA）水平。用纯化的犬血清淀粉样蛋白 A（SAA）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入血清淀粉样蛋白 A（SAA） ，再与 HRP 标记的血清淀粉样蛋白 A（SAA）抗体结合，形成抗体-抗原- 酶标抗体复合物，经过彻底洗涤后加底物 TMB 显色。TMB 在 HRP 酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和

2、样品中的血清淀粉样蛋白 A（SAA ）呈正相关。用酶标仪在 450nm 波长下测定吸光度（OD 值） ，通过标准曲线计算样品中犬血清淀粉样蛋白 A（SAA）浓度。试剂盒组成：试剂盒组成 48 孔配置 96 孔配置 保存说明书 1 份 1 份封板膜 2 片（48） 2 片（96）密封袋 1 个 1 个酶标包被板 148 196 2-8 保存标准品：540g/L 0.5ml1 瓶 0.5ml1 瓶 2-8 保存标准品稀释液 1.5ml1 瓶 1.5ml1 瓶 2-8 保存酶标试剂 3 ml1 瓶 6 ml1 瓶 2-8 保存样品稀释液 3 ml1 瓶 6 ml1 瓶 2-8 保存显色剂 A 液 3

3、 ml1 瓶 6 ml1 瓶 2-8 保存显色剂 B 液 3 ml1 瓶 6 ml1 瓶 2-8 保存终止液 3ml1 瓶 6ml1 瓶 2-8 保存20 倍浓缩洗涤液 20ml1 瓶 30ml1 瓶 2-8 保存样本处理及要求：1. 血清：室温血液自然凝固 10-20 分钟，离心 20 分钟左右（2000-3000 转/分） 。仔细收集上清，保存过程中如出现沉淀，应再次离心。2. 血浆：应根据标本的要求选择 EDTA 或柠檬酸钠作为抗凝剂，混合 10-20 分钟后，离心20 分钟左右（2000-3000 转/ 分） 。仔细收集上清，保存过程中如有沉淀形成，应该再次离心。3. 尿液：用无菌管收

4、集，离心 20 分钟左右（2000-3000 转/分） 。仔细收集上清，保存过程中如有沉淀形成，应再次离心。胸腹水、脑脊液参照实行。4. 细胞培养上清：检测分泌性的成份时，用无菌管收集。离心 20 分钟左右（2000-3000 转2/分） 。仔细收集上清。检测细胞内的成份时，用 PBS（PH7.2-7.4）稀释细胞悬液，细胞浓度达到 100 万/ml 左右。通过反复冻融，以使细胞破坏并放出细胞内成份。离心 20分钟左右（2000-3000 转/分） 。仔细收集上清。保存过程中如有沉淀形成，应再次离心。5. 组织标本：切割标本后，称取重量。加入一定量的 PBS，PH7.4。用液氮迅速冷冻保存备用

5、。标本融化后仍然保持 2-8的温度。加入一定量的 PBS（PH7.4） ，用手工或匀浆器将标本匀浆充分。离心 20 分钟左右（2000-3000 转/分） 。仔细收集上清。分装后一份待检测，其余冷冻备用。6. 标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可将标本放于-20保存，但应避免反复冻融.7. 不能检测含 NaN3 的样品，因 NaN3 抑制辣根过氧化物酶的（HRP ）活性。操作步骤1. 标准品的稀释与加样：在酶标包被板上设标准品孔 10 孔，在第一、第二孔中分别加标准品 100l，然后在第一、第二孔中加标准品稀释液 50l，混匀；然后从第一孔、

6、第二孔中各取 100l分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液50l，混匀；然后在第三孔和第四孔中先各取 50l弃掉，再各取 50l分别加到第五、第六孔中，再在第五、第六孔中分别加标准品稀释液 50ul，混匀；混匀后从第五、第六孔中各取 50l分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液50l，混匀后从第七、第八孔中分别取 50l加到第九、第十孔中，再在第九第十孔分别加标准品稀释液 50l，混匀后从第九第十孔中各取 50l弃掉。 （稀释后各孔加样量都为 50l，浓度分别为 360g/L，240g/L ，120g/L，60g/L， 30g/L） 。2. 加样：分

7、别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同） 、待测样品孔。在酶标包被板上待测样品孔中先加样品稀释液 40l，然后再加待测样品10l（样品最终稀释度为 5 倍） 。加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀。3. 温育：用封板膜封板后置 37温育 30 分钟。4. 配液：将 20 倍浓缩洗涤液用蒸馏水 20 倍稀释后备用。5. 洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置 30 秒后弃去，如此重复 5 次，拍干。6. 加酶：每孔加入酶标试剂 50l，空白孔除外。7. 温育：操作同 3。8. 洗涤：操作同 5。9. 显色：每孔先加入显色剂 A50l，再

8、加入显色剂 B50l，轻轻震荡混匀，37避光显色15 分钟. 10. 终止：每孔加终止液 50l，终止反应（此时蓝色立转黄色） 。11. 测定：以空白空调零，450nm 波长依序测量各孔的吸光度（OD 值） 。 测定应在加终止液后 15 分钟以内进行。注意事项：1 试剂盒从冷藏环境中取出应在室温平衡 15-30 分钟后方可使用，酶标包被板开封后如未用完，板条应装入密封袋中保存。2 浓洗涤液可能会有结晶析出，稀释时可在水浴中加温助溶，洗涤时不影响结果。3 各步加样均应使用加样器，并经常校对其准确性，以避免试验误差。一次加样时间最好控制在 5 分钟内，如标本数量多，推荐使用排枪加样。4 请每次测定

9、的同时做标准曲线，最好做复孔。如标本中待测物质含量过高（样本 OD3值大于标准品孔第一孔的 OD 值） ，请先用样品稀释液稀释一定倍数（ n 倍）后再测定，计算时请最后乘以总稀释倍数（n5） 。5 封板膜只限一次性使用，以避免交叉污染。6 底物请避光保存。7 严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准.8 所有样品，洗涤液和各种废弃物都应按传染物处理。9 本试剂不同批号组分不得混用。10. 如与英文说明书有异，以英文说明书为准。计算：以标准物的浓度为横坐标，OD 值为纵坐标， 在坐标纸上绘出标准曲线，根据样品的 OD 值由标准曲线查出相应的浓度；再乘以稀释 倍数；或用标准物的浓

10、度与 OD 值计算出标 准曲线的直线回归方程式，将样品的 OD 值 代入方程式，计算出样品浓度，再乘以稀释 倍数，即为样品的实际浓度。 （此图仅供参考）试剂盒性能：1.样品线性回归与预期浓度相关系数 R 值为 0.990 以上。2.批内与批见应分别小于 9%和 11%检测范围： 15g/L -480g/L保存条件及有效期：1.试剂盒保存：；2-8。2有效期：6 个月4Canine Serum amyloid AFOR RESEARCH USE ONLYDrug NamesGeneric Name： Canine Serum amyloid A (SAA) ELISA Kit.PurposeTh

11、is kit allows for the determination of SAA concentrations in Canine serum, blood plasma, and other biological fluids.Principle of the assayThe kit assay Canine SAA level in the sample，use Purified Canine SAA antibody to coat microtiter plate wells, make solid-phase antibody, then add SAA to wells, C

12、ombined SAA antibody which With HRP labeled, beome antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is

13、measured spectrophotometrically at a wavelength of 450 nm. The concentration of SAA in the samples is then determined by comparing the O.D. of the samples to the standard curve.5Materials provided with the kitMaterials provided with the kit 48determinations 96 determinations StorageUser manual 1 1Cl

14、osure plate membrane 2 2Sealed bags 1 1Microelisa stripplate 1 1 2-8Standard： 540g/L 0.5ml1 bottle 0.5ml1 bottle 2-8Standard diluent 1.5ml1 bottle 1.5ml1 bottle 2-8HRP-Conjugate reagent 3ml1 bottle 6ml1 bottle 2-8Sample diluent 3ml1 bottle 6ml1 bottle 2-8Chromogen Solution A 3ml1 bottle 6ml1 bottle

15、2-8Chromogen Solution B 3ml1 bottle 6ml1 bottle 2-8Stop Solution 3ml1 bottle 6ml1 bottle 2-8wash solution 20ml1 bottle 30ml1 bottle 2-8Specimen requirements1. serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation

16、 appeared, Centrifugal again.2. plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.3. Urine-collect sue a sterile container, centrifugation 20-min at the

17、speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.4. cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r

18、.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant

19、, If precipitation appeared, Centrifugal again.65. Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8 after melting,add PBS（PH7.4） , Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000

20、r.p.m. remove supernatant.6. extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it cant, specimen can be kept in -20 to preserve, Avoid repeated freeze-thaw cycles.7. Cant detect the sa

21、mple which contain NaN3, because NaN3 inhibits HRP active.Assay procedure1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100l to the first and the second well, then add Standard dilution 50l to the first and the second well, mix; take out 100l form

22、 the first and the second well then add it to the third and the forth well separately. then add Standard dilution 50l to the third and the forth well ,mix ; then take out 50l from the third and the forth well discard, add 50l to the fifth and the sixth well ,then add Standard dilution 50l to the fif

23、th and the sixth well, mix ; take out 50l from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50l to the seventh and the eighth well ,mix ; take out 50l from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dil

24、ution 50l to the ninth and the tenth well, mix , take out 50l from the ninth and the tenth well discard(add Sample 50l to each well after Diluting ,(density: 360g/L，240g/L ，120g/L，60g/L， 30g/L)2.add sample： Set blank wells separately (blank comparison wells dont add sample and HRP-Conjugate reagent,

25、 other each step operation is same). testing sample well. add Sample dilution 40l to testing sample well, then add testing sample 10l (sample final dilution is 5-fold), add sample to wells , dont touch the well wall as far as possible, and Gently mix.3.Incubate: After closing plate with Closure plat

26、e membrane ,incubate for 30 min at 37.4.Configurate liquid: wash solution diluted 20-fold with distilled water and reserve.5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.76.add enzyme：Add

27、HRP-Conjugate reagent 50l to each well, except blank well. 7.incubate：Operation with 3.8.washing：Operation with 5.9.color： Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 3710.Stop the reaction：Add Stop Solution50l to each well, Stop th

28、e reaction(the blue color change to yellow color).11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.Important notes1. The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coat

29、ed if has not use up after opened, the plate should be stored in Sealed bag.2. washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.3. add Sample with sampler Each step, And proofread its accuracy frequently, avoid

30、s the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .4. if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilutio

31、n factor.（ n5）.5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.6. The substrate evade the light preservation.7. Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.8. All samples, washi

32、ng buffer and each kind of reject should according to infective material process.9. Do not mix reagents with those from other lots.8CalculateAssay range15g/L -480g/LStorage and validity1Storage： 2-8.2validity： six months.Take the standard density as the horizontal, the OD value for the vertical ,dra

33、w the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.This chartis for reference only