1、毕 业 论 文 （ 设 计 ） 任 务 书表 1姓 名 专业 水产养殖学 班级 A07 养殖题 目饲料中不同蛋氨酸水平对真鲷（Pagrus major）体氨基酸组成及保留影响的研究毕业论文（设计）计划任务（目的、任务、计划进度、措施、预期结果）：目的：确定真鲷幼鱼的最适蛋氨酸需要量。任务:1.评价饲料中不同的蛋氨酸水平对真鲷体氨基酸组成的影响2.评价饲料中不同的蛋氨酸水平对真鲷体氨基酸保留的影响计划进度：1.2010 年 6 月 5 日开始讨论选题；2.2010 年 6 月 6 日开始查阅资料；3.2010 年 6 月 14 日开始设计实验饲料配方并进行配制饲料；4.2010 年 7 月 15
2、 日开始进行养殖实验；5.2010 年 9 月 30 日实验结束取样；6.2010 年 10 月 1 日开始测试样品，并分析数据；7.2011 年 1 月 8 日开始撰写论文；8.2011 年 5 月 17 日定稿。措施1.用计算机和互联网通过中国期刊网查阅文献和资料2.用营养学的知识进行配方设计3.用 CD4x1TS 多功能催化剂成型机进行实验饲料制作4.在西闪岛 300L 的养殖系统进行饲养实验5.用凯氏定氮仪，脂肪抽提仪、鼓风干燥机，马福炉机器分别测定粗蛋白，粗脂肪水份和灰分6 用 SPSS 软件进行数据分析统计预期结果：能够得出真鲷幼鱼的最适蛋氨酸需要量参 考 资 料1 Abimora
3、d, E.G.,等, Dietary supplementation of lysine and/or methionine on performance, nitrogen retention and excretion in pacu Piaractus mesopotamicus reared in cages. Aquaculture, 2009. 295(3-4): p. 266-270.2 Ahmed, I., M. Khan,和 A.K. Jafri, Dietary methionine requirement of fingerling Indian major carp,
4、iCirrhinus mrigala/i (Hamilton). Aquaculture International, 2003. 11(5): p. 449-462.3 Alam, 等, Methionine requirement of juvenile Japanese flounder Paralichthys olivaceus estimated by the oxidation of radioactive methionine. Aquaculture Nutrition, 2001. 7(3): p. 201-209.4 Berge, G.E., 等, Intestinal
5、absorption of amino acids in fish: kinetics and interaction of the in vitro uptake of -methionine in Atlantic salmon (Salmo salar L.). Aquaculture, 2004. 229(1-4): p. 265-273.5 Chi, S.Y.,等 , Effects of supplementation of crystalline or coated methionine on growth performance and feed utilization of
6、the pacific white shrimp, Litopenaeus vannamei. Aquaculture Nutrition, 2009: p. no-no.6 Espe, M., 等, Methionine intake affect hepatic sulphur metabolism in Atlantic salmon, Salmo salar. Aquaculture, 2008. 274(1): p. 132-141.7 Gibson Gaylord, T., 等, Supplementation of taurine and methionine to all-pl
7、ant protein diets for rainbow trout (Oncorhynchus mykiss). Aquaculture, 2007. 269(1-4): p. 514-524.8 Goff, J.B. 和 D.M. Gatlin Iii, Evaluation of different sulfur amino acid compounds in the diet of red drum, Sciaenops ocellatus, and sparing value of cystine for methionine. Aquaculture, 2004. 241(1-4
8、): p. 465-477.9 Hansen, A.C.,等, Do plant-based diets for Atlantic cod (Gadus morhua L.) need additions of crystalline lysine or methionine? Aquaculture Nutrition, 2010: p. no-no.10 Hidalgo, F., E. Alliot,和 H. Thebault, Methionine- and cystine-supplemented diets for juvenile sea bass (Dicentrarchus l
9、abrax). Aquaculture, 1987. 64(3): p. 209-217.11 Hidalgo, F., M. Kentouri, 和 P. Divanach, Sur lutilisation du self feeder comme outil depreuve nutritionnelle du loup, Dicentrarchus labrax - Rsultats prliminaires avec la mthionine. Aquaculture, 1988. 68(2): p. 177-190.12 Hu, M., 等, Replacement of fish
10、 meal by rendered animal protein ingredients with lysine and methionine supplementation to practical diets for gibel carp, Carassius auratus gibelio. Aquaculture, 2008. 275(1-4): p. 260-265.13 Huang, C.-H. 和 W.-Y. Lin, Estimation of optimal dietary methionine requirement for softshell turtle, Pelodi
11、scus sinensis. Aquaculture, 2002. 207(3-4): p. 281-287.14 Jackson, A.J. 和 B.S. Capper, Investigations into the requirements of the tilapia Sarotherodon mossambicus for dietary methionine, lysine and arginine in semi-synthetic diets. Aquaculture, 1982. 29(3-4): p. 289-297.15 Kim, K.-I., T.B. Kayes,和
12、C.H. Amundson, Requirements for sulfur amino acids and utilization of D-methionine by rainbow trout (Oncorhynchus mykiss). Aquaculture, 1992. 101(1-2): p. 95-103.16 Kwon, Y.M., 等, The effect of synthetic lysine and methionine on in vitro protein synthesis of weaning pigs. Nutrition Research, 1995. 1
13、5(3): p. 429-437.17 Li, M.H. 和 E.H. Robinson, Effects of supplemental lysine and methionine in low protein diets on weight gain and body composition of young channel catfish Ictalurus punctatus. Aquaculture, 1998. 163(3-4): p. 297-307.18 Liebert, F.和 K. Benkendorff, Modelling of threonine and methio
14、nine requirements of Oreochromis niloticus due to principles of the diet dilution technique. Aquaculture Nutrition, 2007. 13(6): p. 397-406.19 Luo, Z.,等, Dietary l-methionine requirement of juvenile grouper Epinephelus coioides at a constant dietary cystine level. Aquaculture, 2005. 249(1-4): p. 409
15、-418.20 Mai, K., 等, Dietary methionine requirement of large yellow croaker, Pseudosciaena crocea R. Aquaculture, 2006. 253(1-4): p. 564-572.21 Michael, F.R., 等, Effect of choline and methionine as methyl group donors on juvenile kuruma shrimp, Marsupenaeus japonicus Bate. Aquaculture, 2006. 258(1-4)
16、: p. 521-528.22 Millamena, O.M., M.N. Bautista-Teruel, 和 A. Kanazawa, Methionine requirement of juvenile tiger shrimp Penaeus monodon Fabricius. Aquaculture, 1996. 143(3-4): p. 403-410.23 Monroig, ., 等, Enrichment of Artemia nauplii in vitamin A, vitamin C and methionine using liposomes. Aquaculture
17、, 2007. 269(1-4): p. 504-513.24 Murai, T., W. Daozun 和 H. Ogata, Supplementation of methionine to soy flour diets for fingerling carp, Cyprinus carpio. Aquaculture, 1989. 77(4): p. 373-385.25 Nordrum, S., 等, Effects of methionine, cysteine and medium chain triglycerides on nutrient digestibility, ab
18、sorption of amino acids along the intestinal tract and nutrient retention in Atlantic salmon (Salmo salar L.) under pair-feeding regime. Aquaculture, 2000. 186(3-4): p. 341-360.26 Rollin, X., 等, Quantitative methionine requirement of Atlantic salmon (Salmo salar L.) juveniles. Aquaculture, 1994. 124
19、(1-4): p. 61-61.27 Ruchimat, T.,等, Quantitative methionine requirement of yellowtail (Seriola quinqueradiata). Aquaculture, 1997. 150(1-2): p. 113-122.28 Sardar, P., 等., Effect of dietary lysine and methionine supplementation on growth, nutrient utilization, carcass compositions and haemato-biochemi
20、cal status in Indian Major Carp, Rohu (Labeo rohita H.) fed soy protein-based diet. Aquaculture Nutrition, 2009. 15(4): p. 339-346.29 Schwarz, F.J., M. Kirchgessner, 和 U. Deuringer, Studies on the methionine requirement of carp (Cyprinus carpio L.). Aquaculture, 1998. 161(1-4): p. 121-129.30 Segovia
21、-Quintero, M.A. 和 R.C. Reigh, Coating crystalline methionine with tripalmitin-polyvinyl alcohol slows its absorption in the intestine of Nile tilapia, Oreochromis niloticus. Aquaculture, 2004. 238(1-4): p. 355-367.31 Simmons, 等, Dietary methionine requirement of juvenile Arctic charr Salvelinus alpi
22、nus (L.). Aquaculture Nutrition, 1999. 5(2): p. 93-100.32 Sveier, 等, Dietary inclusion of crystalline D- and L-methionine: effects on growth, feed and protein utilization, and digestibility in small and large Atlantic salmon (Salmon salar L.). Aquaculture Nutrition, 2001. 7(3): p. 169-181.33 Tan, B.
23、 和 K. Mai, Zinc methionine and zinc sulfate as sources of dietary zinc for juvenile abalone, Haliotis discus hannai Ino. Aquaculture, 2001. 192(1): p. 67-84.34 Tang, L., 等., Effect of methionine on intestinal enzymes activities, microflora and humoral immune of juvenile Jian carp (cyprinus carpio var. Jian). Aquaculture Nutrition, 2009. 15(5): p. 477-483.35 Teshima, S., A. Kanazawa, S. Koshio, Supplemental effects of methionine-enriched plastein in Penaeus japonicus diets. Aquaculture, 1992. 101(1-2): p. 85-93. 教研室意见：指导小组组长或教研室负责人（签名）： 年 月 日
