1、HPLC-ELSD 法测定黄芪提取物中黄芪甲苷的含量吴永平，徐永梅，曹园，曹正中(江苏省药物研究所，江苏南京 210009)关键词：HPLC-ELSD；黄芪提取物；黄芪甲苷；喷雾干燥摘要：目的： 研究喷雾干燥法制备黄芪提取物中黄芪甲苷的 HPLC-ELSD 测定方法。方法：黄芪醇提液经浓缩后喷雾干燥，采用蒸发光散射检测器(ELSD)以黄芪甲苷为对照品对黄芪提取物中的黄芪甲苷进行 HPLC 分析，色谱柱：Elite-ODS，流动相乙腈-水(3664)，流速：1.0ml/min,ELSD 参数：漂移管温度为 105C，N2 流速为 2.84ml/min。结果：按选定的色谱条件测定，线性良好，平均加
2、样回收率达 91.68%,RSD 为 1.64%，实验还发现喷雾干燥过程中加入可溶性淀粉有助于粉末形成，但对含量测定有影响。结论：本方法对黄芪甲苷的测定良好，应寻找更好的喷雾干燥助剂。中图分类号：R284.2 文献标识码： A 文章编号：1001-1528(2001)-0673-02Determination of Astragloside IV in Extraction of Radix Astragali by HPLC-ELSDWU Yong-ping,XU Yong-mei,CAO Yuan,CAO Zheng-zhong (Jiangsu Provincial Institute
3、of Materia Medica, Jiangsu Nanjing 210009, China)Key words： HPLC-ELSD; extractive of Radix Astragali; Astragaloside IV; spray-dryAbstract： Objective： To develop a method to determine Astragaloside IV in spray-dry of Rad ix Astragali by HPLC-ELSD. Methods： ELSD was used to determine directly Astragal
4、oside IV in sp ray-dry of Radix Astragali on Elite-OSD column, using CHCN-H （3664） as a mobile phase. The flow rate was 1.0 ml/min. The temperature of drift tube for ELSD was 105C and the flow rate of N2 was 2.84 ml/min. Results： The recovery of the added sample was 91.68% and RSD 1.6 4 %. Soluble amylum was helpful for spray-dry but it can influence was determinat ion of Astragaloside IV. Conclusion： The method is accurate and can be used in the determination of Astragaloside IV in spray-dry of Radix Astragali. Better adjuvant should be found.
