IV-GeneandstemcelltechnologyGenetechnology GeneClone GeneAnalysisandIdentification GeneTransformation GeneEditingGenecloneGenelibraryGenomicDNAmRNADNAfragmentscDNARestrictionenzymeDNAlibcDNAlibligationligationReversetranscriptionvectorToolsforgeneclone Restrictionendonuclease DNApolymeraseandDNAligase Vector:plasmidor l-phage Hostcells PCRandRT-PCRPCR(PolymeraseChainReaction)cDNA 合成与RT-PCRMultiple Annealing and Looping Based Amplification Cycles(MALBAC)forSinglecellgenomicsequencing高通量DNA 测序DNAa