1、连接互相匹配的粘性末端，产生新的酶切位点通常连接互相匹配的粘性末端可以产生新的酶切位点。以下组合是通过计算机程序计算出来的，尽管我们希望能尽量保证其准确性，但有些未经实验证实，因此不能保证 100%的可行性。如果有同裂酶存在，则只列出其中的一个酶。本表中列出的内切酶均为已商品化的内切酶。识别序列不够严谨的内切酶（例如，识别多个序列的酶）只在括号内标出相关的序列，但注意这些酶不只切割该序列。“-“表示连接产物不能被再切割。酶 连接到 可进行切割的酶Ban I (G/GTACC) Acc65 I, Ban I, Kpn I, Nla IV, Rsa I Acc65 I (G/GTACC) BsiW
2、 I, BsrG I Rsa IAcc I(GT/CGAC) Aci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I -(GT/CGAC) Cla I, BstB I, Taq I Taq IAcc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I -BsaH I (GR/CGCC), HinP1 I, Nar I Aci IAci I(C/CGC) Hpa II Hpa IIAcl I (AA/CGTT)Acc I (GT/CGAC), Aci I, Cla I, BstB I, HinP1 I, Hpa
3、II, Nar I, Taq I -Afl III(A/CGCGT) Asc I, BssH II BstU I, Hha I(A/CATGT) BspH I, Nco I, Pci I Nla III(A/CGCGT) Mlu I Afl III, BstU I, Mlu IAva I (C/CCGGG), Xma I Hpa II, Nci I, ScrF IBsaW I, BspE I BsaW I, Hpa IIBsrF I (A/CCGGT), SgrA I (CA/CCGGTG) Age I, BsaW I, BsrF I, Hpa IIAge I (A/CCGGT)NgoM IV
4、 BsrF I, Hpa IIApa I (GGGCC/C)Ban II (GGGCC/C), Bsp1286 I (GGGCC/C) Apa I, Ban II, Bsp120 I, Bsp1286 I, Hae III, Nla IV, Sau96 IApaL I (G/TGCAC)Sfc I (C/TGCAG) Bsg IApo I(A/AATTY) EcoR I Apo I, Tsp509 I(G/AATTY) EcoR I Apo I, EcoR I, Tsp509 I(R/AATTY) Mfe I, Tsp509 I Tsp509 IAfl III (A/CGCGT), Mlu I
5、 BstU I, Hha IAsc I (GG/CGCGCC) BssH II BssH II, BstU I, Cac8 I, Hha IBfa I, Csp6 I, Nde I -Ase I (AT/TAAT) Mse I Mse IBsiE I (CGAT/CG) Dpn II, Pvu IPac I Mse IAsiS I(GCGAT/CGC) Pvu I Dpn II, Pvu IAva I(C/CCGGG) Age I, BsaW I, BspE I, BsrF I (R/CCGGY), NgoM IV, SgrA I (CR/CCGGYG)Hpa II, Nci I, ScrF
6、I(C/TCGAG) Xho I Ava I, Taq I, Xho I(C/TCGAG) Sal I Taq I(C/CCGGG) Xma I Ava I, BsaJ I, Hpa II, Nci I, ScrF I, Sma IPpuM I (RG/GACCY) Ava II, Nla IV, Sau96 IRsr II Ava II, Sau96 IAva II (G/GWCC)PpuM I (RG/GTCCY) Ava II, BsmF I, Nla IV, Sau96 INhe I, Spe I, Xba I Bfa IAvr II (C/CTAGG) Sty I (C/CTAGG)
7、 Avr II, Bfa I, BsaJ I, Sty IBcl I, Dpn II Alw I, Dpn IIBgl II, BstY I (R/GATCY) Alw I, BstY I, Dpn IIBamH I (G/GATCC)BstY I (G/GATCC) Alw I, BamH I, BstY I, Dpn II, Nla IVBan I(G/GTACC) Acc65 I Acc65 I, Ban I, Kpn I, Nla IV, Rsa I(G/GCGCC) Kas I Ban I, BsaH I, Hae II, Hha I, Kas I, Nar I, Nla IV(G/
8、GTACC) BsiW I, BsrG I Rsa IBan II(GGGCC/C) Apa I, Bsp1286 I (GGGCC/C) Apa I, Ban II, Bsp1286 I, Hae III, Nla IV, Sau96 I(GAGCT/C) Bsp1286 I (GAGCT/C), Sac I Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac IBamH I, BstY I (R/GATCY) Alw I, Dpn IIBcl I(T/GATCA) Bgl II, Mbo II Dpn IIBfa I (C/TAG)Ase I, Csp6 I,
9、Mse I, Nde I -BamH I, BstY I (R/GATCY) Alw I, BstY I, Dpn IIBgl II (A/GATCT) Bcl I, Dpn II Dpn IIBsaH I(GR/CGYC) Acc I (GT/CGAC), Cla I, BstB I, Taq I -(GA/CGYC) Aci I, HinP1 I Hga I(GG/CGYC) Aci I, HinP1 I Hha I(GG/CGYC) Hpa II Aci I(GA/CGYC) Nar I BsaH I, Hga I(GG/CGYC) Nar I Ban I, BsaH I, Hae II
10、, Hha I, Nar I, Nla IVAge I, BsrF I (R/CCGGY), SgrA I (CR/CCGGYG) Age I, BsaW I, BsrF I, Hpa IIAva I (C/CCGGG), Xma I Hpa II, Nci I, ScrF IBspE I BsaW I, BspE I, Hpa IIBsrF I (R/CCGGY), NgoM IV BsrF I, Hpa IIBsaW I (W/CCGGW)NgoM IV Hpa IIBsiE I(CGAT/CG) Pac I Mse I(CGAT/CG) Pvu I BsiE I, Dpn II, Pvu
11、 I(CGGC/CG) Sac II Aci IBsp1286 I (GTGCA/C) BsiHKA I, Bsp1286 IBsp1286 I (GAGCA/C) BsiHKA I, Bsp1286 IBsp1286 I (GAGCT/C), Sac I Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac INsi I -BsiHKA I(GTGCA/C) Pst I, Sbf I Bsg IBsiW I (C/GTACG)Acc65 I, Ban I (G/GTACC), BsrG I Rsa IBsp1286 I(GGGCC/C) Apa I, Ban II
12、(GGGCC/C) Apa I, Ban II, Bsp1286 I, Hae III, Nla IV, Sau96 I(GTGCA/C) BsiHKA I ApaL I, BsiHKA I, Bsp1286 I(GGGCC/C) Ban II (GGGCC/C) Ban II, Bsp1286 I(GAGCT/C) Ban II (GAGCT/C), BsiHKA I, Sac I Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac I(GWGCW/C) BsiHKA I BsiHKA I, Bsp1286 I(GTGCA/C) Nsi I -(GTGCA/C)
13、Pst I, Sbf I Bsg IAge I, BsaW I, BsrF I (R/CCGGY), SgrA I (CR/CCGGYG) BsaW I, Hpa IIAva I (C/CCGGG), Xma I Hpa II, Nci I, ScrF IBsaW I BsaW I, BspE I, Hpa IIBspE I (T/CCGGA)BsrF I (R/CCGGY), NgoM IV Hpa IIBspH I Afl III (A/CATGT), Nco I, Sty I (C/CATGG) Nla III(T/CATGA)BsrF I(A/CCGGY) Age I, BsaW I
14、Age I, BsaW I, BsrF I, Hpa II(G/CCGGY) Age I, BsaW I, NgoM IV BsrF I, Hpa II(R/CCGGY) Ava I (C/CCGGG), Xma I Hpa II, Nci I, ScrF I(A/CCGGY) BsaW I, BspE I BsaW I, Hpa II(R/CCGGY) BsaW I, BspE I Hpa II(G/CCGGY) NgoM IV BsrF I, Cac8 I, Hpa II, Nae I(CR/CCGGYG) SgrA I BsrF I, Hpa IIBsrG I (T/GTACA)Acc6
15、5 I, Ban I (G/GTACC), BsiW I Rsa IAfl III (A/CGCGT), Mlu I BstU I, Hha IBssH II (G/CGCGC) Asc I BssH II, BstU I, Cac8 I, Hha IAcc I (GT/CGAC), Cla I, Taq I Taq IBstB I (TT/CGAA) Aci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I -BstY I(A/GATCY) BamH I, Bgl II Alw I, BstY I, Dpn II(G/GATCY) BamH
16、 I Alw I, BamH I, BstY I, Dpn II, Nla IV(R/GATCY) Bcl I, Dpn II Dpn II(G/GATCY) Bcl I, Dpn II Alw I, Dpn II(A/GATCY) Bgl II Bgl II, BstY I, Dpn IIAcc I (GT/CGAC), BstB I, Taq I Taq ICla I (AT/CGAT) Aci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I -BamH I, BstY I (R/GATCC) Alw I, Dpn IIDpn II/M
17、bo I/ Sau3A I (/GATC) Bcl I, Bgl II, BstY I (R/GATCY) Dpn IIEae I(Y/GGCCR) PspOM I Hae III, Sau96 I(C/GGCCR) Eag I BsiE I, Eae I, Eag I, Hae III(T/GGCCR) Eag I Eae I, Hae III(C/GGCCR) Not I Aci I, BsiE I, Eae I, Eag I, Fnu4H I, Hae III(T/GGCCR) Not I Aci I, Eae I, Fnu4H I, Hae IIIPspOM I Hae III, Sa
18、u96 IEae I (Y/GGCCR) Eae I, Hae IIIEae I (C/GGCCG) BsiE I, Eae I, Eag I, Hae IIIEag I (C/GGCCG)Not I Aci I, BsiE I, Eae I, Eag I, Fnu4H I, Hae IIIApo I (G/AATTC) Apo I, EcoR I, Tsp509 IApo I (R/AATTY) Apo I, Tsp509 IEcoR I (G/AATTC)Mfe I, Tsp509 I Tsp509 IAcc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I
19、 -Aci I, BsaH I (GR/CGCC), Nar I Hha IBsaH I (GR/CGTC) Hga IHinP1 I (G/CGC)Hpa II Aci IAcc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I -Hpa II/Msp I (C/CGG) Aci I, BsaH I (GR/CGCC), HinP1 I, Nar I Aci IKas I (G/GCGCC)Ban I (G/GCGCC) Ban I, BsaH I, Hae II, Hha I, Kas I, Nar I, Nla IVMfe I (C/AATTG)Apo I
20、 (R/ATTTY), EcoR I, Tsp509 I Tsp509 IAfl III (A/CGCGT) Afl III, BstU I, Mlu IMlu I (A/CGCGT) Asc I, BssH II BstU I, Hha IAse I Mse IMse I (T/TAA) Bfa I, Csp6 I, Nde I -Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I -Aci I, HinP1 I Hha IBsaH I (GR/CGCC) Ban I, BsaH I, Hae II, Hha I, Nar I, Nla IVBsaH I
21、 (GR/CGTC) BsaH I, Hga INar I (GG/CGCC)Hpa II Aci IAfl III (A/CATGT), BspH I Nla IIISty I (C/CATGG) BsaJ I, Nco I, Nla III, Sty INco I (C/CATGG)Pci I Nla IIINde I (CA/TATG)Ase I, Bfa I, Csp6 I, Mse I -Age I, BsaW I, BsrF I (R/CCGGY), SgrA I BsrF I, Hpa IINgoM IV (G/CCGGC) Ava I (C/CCGGG), Xma I Hpa
22、II, Nci I, ScrF IBsaW I, BspE I Hpa IIBsrF I (R/CCGGC), SgrA I BsrF I, Cac8 I, Hpa II, Nae INhe I (G/CTAGC)Avr II, Spe I, Sty I (C/CTAGG), Xba I Bfa INla III (CATG/)Sph I, Nsp I Nla IIIPspOM I Aci I, Eae I, Fnu4H I, Hae IIIEag I Aci I, BsiE I, Eae I, Eag I, Fnu4H I, Hae IIINot I (GC/GGCCGC)Eae I (Y/
23、GGCCR) Aci I, BsiE I, Eae I, Fnu4H I, Hae IIINsi I (ATGCA/T)BsiHKA I (GTGCA/C), Bsp1286 I (GTGCA/C), Pst I, Sbf I -Nsp I (RCATG/Y)Nla III, Sph I Nla IIIPac I (TTAAT/TAA)BsiE I (CGAT/CG), Pvu I Mse IPci I (A/CATGT)Afl II (A/CATGT), BspH I, Nco I, Sty I (C/CTAGG) Nla IIIPpuM I(RG/GWCCY) Ava II, Rsr II
24、 Ava II, Sau96 I(GG/GTCCY) Ava II, Rsr II Ava II, BsmF I, Nla IV, Sau96 I(GG/GACCY) Ava II, Rsr II Ava II, Nla IV, Sau96 IPspOM I Eae I (Y/GGCCR), Eag I Hae III, Sau96 I(G/GGCCC) Not I Aci I, Fnu4H I, Hae III, Sau96 IBsiHKA I, Bsp1286 I (GTGCA/C) Bsg INsi I -Pst I (CTGCA/G)Sbf I Pst IAsiS I Dpn I, P
25、vu IPac I Mse IPvu I (CGAT/CG)BsiE I (CGAT/CG) BsiE I, Dpn II, Pvu IAva II, PpuM I (RG/GACCY) Ava II, Sau96 IPpuM I (RG/GACCY) Ava II, Nla IV, Sau96 IRsr II (CG/GWCCG)PpuM I (RG/GTCCY) Ava II, BsmF I, Nla IV, Sau96 ISac I (GAGCT/C)Ban II (GAGCT/C), BsiHKA I, Bsp1286 I (GAGCT/C) Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac ISac II (CCGC/GG)BsiE I (CGGC/CG) Aci ISal I (G/TCGAC)Ava I (C/TCGAG), Xho I Taq IBsiHKA I, Bsp1286 I (GTGCA/C) Bsg INsi I -Sbf I (CCTGCA/GG)Pst I Pst ISfc I (C/TGCAG)ApaL I Bsg ISgrA I See BsrF I
