1、BIOSTAT BPLUS-CC 5升 多功能组合式生物发酵 罐 一、主机技术参数描述 BIOSTAT Bplus-CC 5 L Exclusive Flow; 230 VAC for cell culture Basic Unit with Micro DCU controller - bench top housing, dimensions (WxHxD) 320 x 730 x 565 mm Built into Basic Unit - Digital Fermentor Control-Board - Amplifiers for: * Temperature * pH * pO2
2、 * Foam * Level - Operator interface * Graphical user interface with touch screen - Stirrer drive * maintenance free Servo drive 200 W * digital speed measurement * speed range at aeration of 0,5 vvm 1 l UniVessel: 20 - 2000 rpm 2 l UniVessel: 20 - 2000 rpm 5 l UniVessel: 20 - 1500 rpm 10 l UniVesse
3、l: 20 - 800 rpm - Temperature control system * for temperatures from 80C to 8C above cooling water * electrical heater 1000 W * pulsed cooling valve * circulation pump - Aeration system “Exclusive Flow“ * Two impendent gas out lets for “Sparger“ and “head space aeration“ * Rotameter Spager 0,01-0,16
4、6 l/min * Rotameter head space aeration 0,42-4,2 l/min * Gas mixing of AIR, O2, N2, CO2 for “Sparger“, AIR for “head space aeration“ * 3 x 3/2-solenoid valve for gas mixing station for air, O2, N2, CO2 * 2 x check valve * 2 x safety valve * controlled gases required 0,8 bar g - four configurable per
5、istaltic pumps Scope of software function BIOSTAT B plus: Current software version with standard functions - Graphical process displays - Digital calibration of sensors for: * pH sensor, incl. recalibration * pO2 sensor - Totalizers for: * Acid pump * Base pump * Antifoam pump * Level pump * Substra
6、te A pump * Substrate B pump - Digital controllers for: * Temperature (PID controller) * Stirrer speed (Set point controller) * pH (PID controller) * pO2 (2 stage PID cascade controller) * Airflow (Set point controller) * Antifoam * Level (alternatively via probe or balance) * Gasmix (Set point cont
7、roller) * Substrate A (Set point controller) * Substrate B (Set point controller) - Substrate controller can be configured to an external analog pump or to an unused integrated pump - Set point profile for substrate controller - Safety shut down for: * Heating at temperature measurement failure - Pa
8、ssword protection for all system critical parameters - Manual override of all process outputs - Balance connection RS 232 - Printer connection via serial interface RS 232 - Communication with supervisory computer via standard serial interface RS232 ASCII-Protocol - optional RS422 can be choosen Powe
9、r supply: 230 VAC/50-60 Hz UniVessel Total volume 6,6 L Working volume 0,4 - 5,0 L Consists of: - jacketed glass vessel with round bottom - stainless steel top plate with vertical handles - stainless steel stand - removable tray for storage vessels (3 x 500 mL) - 3 x inlet and exhaust filter - harve
10、st pipe, height adjustable - addition-/ inoculation port - 4-way addition port - universal adapter - exhaust cooler - temperature sensor Pt 100 - pH-electrode - pO2-electrode - foam probe - level probe - 3 x storage bottle, 500 ml - aeration tube with micro sparger (20 m) - stirrer shaft with single
11、 mechanical seal - 3-blade segment impeller (angle adjustable) - manual sampler Material: - product side * stainless steel 1.4435 * micro sparger frit 1.4404 * borosilicat glass * EPDM / FDA - others * stainless steel 1.4301 Required inner autoclave dimensions: - height 700 mm - diameter 300 mm MFCS
12、/DA MFCS/DA-1 Software Kit - SCADA-Software for * data acquisition * process visualization * process control * evaluation * data export - incl. programmable interface - single user license for connection of 1 fermentor with DCU via RS 232 interface - incl. standard configurations for BIOSTAT-ferment
13、ors - cable for RS 232 connection - delivered on CD-ROM - necessary operating system Microsoft Windows2000/WindowsXP 二、配件 （附件） 描述 1、 Cat. No. 884463/1 ： Inoculation port B(接种口 ) 2、 Cat. No. TBA Spinfilter B5 plus, 10 um(细胞连续旋转过滤器 ) - Mounted on stirrer shaft - pore size 10 um - made of stainless ste
14、el mat. no. 1.4404 3、 Cat. No. 880827/9 ： Harvets tube SF B5(取料管 ) 4、 Cat. No. TBA： Harvets tube B5 WITH FILTER(取料管 ) 5、 Cat. No. 882364/2 ： Storage/harvest bottle 10 L(贮 /收料瓶 ) - made of polypropylene and autocalvable - with stainless steel head piece with 3 top plate ports - for 4 x 7 mm tubing -
15、silicone tubing 4 x 7 mm, length 40 cm - top plate connector with gasket 6、 Cat. No. 881007/9： Plastic filter(空气过滤器 ) 7、 Cat. No. 880924/0 (防污染快接口 )： STT-female connector for 4x7 mm silicone tubing 8、 Cat. No. 880920/8 (防污染快接口 )： STT-male connector for 4x7 mm silicone tubing 9、 Cat. No. 880926/7 ： S
16、lot membrane of latex for STT connector(接口膜 ) 10、 Cat. No. 3997141/4 ： Silicone tubing 3.2 x 1.6 mm(硅胶管 ) 11、 Cat. No. 3997108/2 ： Silicone tubing 1.6 x 1.6 mm(硅胶管 ) 12、 Cat. No. 880941/1(防污染快接口 )： STT-female connector for silicone tubing 2 x 4 mm 13、 Cat. No. 880940/2(防污染快接口 ) ： STT-male connector
17、for silicone tubing 2 x 4 mm 14、 Cat. No. 3318257/4 ： Spare pH electrode, For BPLUS 5 CC 15、 Cat. No. 3409062/2 ： pO2 membrane kit 16Cat. No. 3922480/5 ： Aeration filter for storage bottles 17Cat. No. 881007/9 ： Plastic filter for aeration 18、 Cat. No. 618201/0 ： Silicone tubing 2 x 4 mm 19： Cat. No
18、. 3997115/5 ： Silicone tubing 4 x 7 mm 20、 Cat. No. TBA： BC5 plus adaptor 21、 Cat. No. 884801/7 ： Gas supply device for culture vessel B5 Plus(无气泡供气系统 ) - for bubble free aeration - insert holder for silicone tubing mounted to the top plate (silicone tubing, 10.4 m, dia. 3 x 3.7 mm) - membrane filte
19、rs for aeration and exhaust gas (Sartorius), 2 pcs. - pressure restriction gauge for installation into the supply unit 22、 Cat. No. TBA ： 6-Blade impellers(搅拌桨 ) 23、 Cat. No. 884748/7： Spinfilter B5 plus, 75 um(细胞连续旋转过滤器 ) - Mounted on stirrer shaft - pore size 75 um - made of stainless steel mat. no. 1.4404 24、 Cat. No.TBA： Ring Sparge(通气管 )
