1、不同内切酶对识别位点以外最少保护碱基数目的要求不同内切酶对识别位点以外最少保护碱基数目的要求切割率% 酶 寡核苷酸序列 2 hr 20 hr Acc I GGTCGACC CGGTCGACCG CCGGTCGACCGG 0 0 0 0 0 0 Afl III CACATGTG CCACATGTGG CCCACATGTGGG 0 90 90 0 90 90 Asc I GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA 90 90 90 90 90 90 Ava I CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA 50 90 90 90 90 90 BamH I
2、CGGATCCG CGGGATCCCG CGCGGATCCGCG 10 90 90 25 90 90 Bgl II CAGATCTG GAAGATCTTC GGAAGATCTTCC 0 75 25 0 90 90 BssH II GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA 0 0 50 0 0 90 BstE II GGGT(A/T)ACCC 0 10 BstX I AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT 0 25 25 0 50 90 Cla I CATCGAT
3、G GATCGATC CCATCGATGG CCCATCGATGGG 0 0 90 50 0 0 90 50 EcoR I GGAATTCC CGGAATTCCG CCGGAATTCCGG 90 90 90 90 90 90 Hae III GGGGCCCC AGCGGCCGCT TTGCGGCCGCAA 90 90 90 90 90 90 Hind III CAAGCTTG CCAAGCTTGG CCCAAGCTTGGG 0 0 10 0 0 75 Kpn I GGGTACCC GGGGTACCCC CGGGGTACCCCG 0 90 90 0 90 90 Mlu I GACGCGTC CG
4、ACGCGTCG 0 25 0 50 Nco I CCCATGGG CATGCCATGGCATG 0 50 0 75 Nde I CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC 0 0 0 0 75 75 0 0 0 0 90 90 Nhe I GGCTAGCC CGGCTAGCCG CTAGCTAGCTAG 0 10 10 0 25 50 切割率%酶 寡核苷酸序列2 hr 20 hrNot I TTGCGGCCGCAA ATTTGCGGCCGCTTT
5、A AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA0 10 10 25 250 10 10 90 90Nsi I TGCATGCATGCA CCAATGCATTGGTTCTGCAGTT10 9090 90Pac I TTAATTAA GTTAATTAAC CCTTAATTAAGG0 0 00 25 90Pme I GTTTAAAC GGTTTAAACC GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGG0 0 0 750 25 50 90Pst I GCTGCAGC T
6、GCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT0 10 90 90 00 10 90 90 0Pvu I CCGATCGG ATCGATCGAT TCGCGATCGCGA0 10 00 25 10Sac I CGAGCTCG 10 10Sac II GCCGCGGC TCCCCGCGGGGA0 500 90Sal I GTCGACGTCAAAAGGCCATAGCGGCCGC GCGTCGACGTCTTGGCCATAGCGGCCGCGG ACGCGTCGACGTCGG
7、CCATAGCGGCCGCGGAA0 10 100 50 75Sca I GAGTACTC AAAAGTACTTTT10 7525 75Sma I CCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA0 0 10 9010 10 50 90Spe I GACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG10 10 0 090 90 50 50Sph I GGCATGCC CATGCATGCATG ACATGCATGCATGT0 0 100 25 50Stu I AAGGCCTT GAAGGCCTTC AAAAGGCCTTTT90 90 9090 90 90Xba I CTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG0 90 75 750 90 90 90Xho I CCTCGAGG CCCTCGAGGG CCGCTCGAGCGG0 10 100 25 75Xma I CCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA0 25 50 900 75 90 90
