1、Modern Biophysical TechniquesAn Introduction,太阳：由64种元素构成；月球：岩样中含66种元素；地球：存在94种元素,生物:核苷酸和氨基酸, 无机物(微量元素),人体内的元素含量分布,Whats this?,What can we use to get information,sound,light,heat,Electricitymagnetic,force,microscope,Atomic Force Microscopy(AFM),原子力显微术,see,feel,curvature radius , 10nm, 1nm,x-y range,
2、16um; x-y resolution, 1nm;z resolution, 0.1nm,control,DAPT,Jagged1,Dendrtic processe of jagged1,Interaction between PrP106-126 and supported lipid bilayer(POPC/Chl),Fibrogenesis,Total Internal Reflection Fluorescence Microscopy (TIRFM),全内反射荧光显微镜,Evanescent Wave Exponential intensity decay,102nm,High
3、 sensitivity from increasing S/N,CLSM: 500nmTIRFM: 200nm,Images of beta amyloid fibrils observed by ThT fluorescence and TIRFM,Individual kinesin-GFP molecules (pseudo-coloured green) moving along Cy5-labelled axonemal microtubules (pseudo-coloured red),Nature,1997,vol.388,338,Surface Plasmon Resona
4、nce(SPR)表面等离子共振技术,等离子体是物质第四态,固体 冰,00C,1000C,100000C,电离气体是一种常见的等离子体,普通气体,等离子体,等离子体通常是指由密度相当高的自由正、负电荷组成的气体,其中正、负带电粒子数目几乎相等,内部不形成空间电荷,金属的价电子可以看成是均匀正电荷背景中运动的电子气体,这实际上也是一种等离子体,当金属受到电磁干扰时,金属中电子密度分布就会变化,导致局部电子密度振荡,并以密度起伏波的形式表现出来。金属中价电子相对于正离子背景的这种振荡与导电气体中的等离子振荡相似,故称为金属中的等离子振荡,楔形光,Process of SPR experiment,M
5、icrocalorimetry,Microcalorimetry,function,structure,energetics,Thermodynamics information,Differential Scanning Calorimetry (DSC)差式扫描量热技术,基本原理图,热天平,功率补偿型(Power Compensation)条件:样品和参比始终保持相同温度参数:外界提供的能量差Q（热量差）,热流型(Heat Flux)条件:外界提供给样品和参比品相同能量参数:样品和参比品两端的温差T,Temperature dependece of excess heat capacity
6、 of CK (creatine kinase ) in the presence of glycerol with concentrations of 0% ,5%,10%,20% and 30%. The enzyme concentration was 9.3M and the temperature scanning rate was 1 K/min,Isothermal Titration Calorimetry(ITC)等温滴定量热技术,纳米探针技术-量子点,Quantum Dots(QDs),Quantum Dots,hn will increase with QDs size decrease,
