1、Drug Resistance Gene Transferred by Plasmid,Plasmid gets out and into the host cell,Resistant Strain,New Resistance Strain,Non-resistant Strain,Plasmid,EnzymeHydrolyzingAntibiotics,mRNA,Juang RH (2004) BCbasics,Target Genes Carried by Plasmid,1 plasmid1 cell,Recombinant PlasmidTransformation,Target
2、GeneRecombination,RestrictionEnzyme,RestrictionEnzyme,Chromosomal DNA,Target Genes,DNA Recombination,Transformation,Host Cells,Juang RH (2004) BCbasics,Amplification and Screening of Target Gene,1,1 cell line, 1 colony,X100,X1,000,PlasmidDuplication,Bacteria Duplication,Plating,Pick the colonycontaining target gene,=100,000,Juang RH (2004) BCbasics,
