1、Hands-On,In-Vivo Gene Delivery using ECM 830 and 2 Needle Array Electrode活体基因转移使用ECM830和 2 Needle Array 电极,Keep the mouse under anesthesia during the procedure麻醉老鼠,Perform IM(intramuscular) injection of desired plasmid in the anterior tibialis muscle.在目的区域，注射所需要的质粒（肌肉注射）,Insert two-needle array elec
2、trode into the muscle. Make sure the needles span the injection site. 将两针电极插入肌肉。（注射点应在电极之间）,While holding the array in place, deliver the pulses to the muscle.释放脉冲（使用脚踏开关，踩一下就好了）,Observations:观察结果,Mouse will twitch slightly while being pulsed脉冲时，老鼠会轻微抖动Return mouse to cage after pulsing. Mouse will resume normal activities within minutes. EP does not cause any side effects on mouse.脉冲后将老鼠放回笼子内，几分钟后老鼠恢复正常的活动，电穿孔不会对老鼠有任何的副作用。,Thats AllFOLKS!,
