1、1,转基因动物平台,医学部中心楼8层,2,仪器设备： 显微操纵系统、倒置显微镜、自制防震台、细胞培养箱、超净台、方波电穿孔系统、高速冷冻离心机和独立的动物饲养室。功能： 构建过表达心血管疾病相关基因载体，利用转基因或基因敲除的方法，制备心血管疾病小鼠和家兔的模式动物，为系统地研究心血管疾病病理机制、评价药物药效和药理提供体内研究的技术手段。,3,Genetically Engineered Mouse Models:Transgenic characterize the ability of a promoter to direct tissue-specific gene expressio
2、n examine the effects of overexpressing andmisexpressing endogenous or foreign genes at specific times and locations in the animals,4,5,内皮细胞Tie2,平滑肌细胞细胞SM22a,神经系统Neuron specific enolase,nestin, synapsin, GFAP,肝脏Albumin, ApoE,脂肪AP2,肾脏Uromodulin, mSGLT2,mKAP2, CYP27B1,皮肤Keratin5,keratin6,心脏MHC, MLC2,T
3、ransgene Design: Representative Examples of Tissue-Specific Expression Profiles,全身表达EF1，CMV,胰腺Elastase,赢润生物（Yrbio）长沙市国家高新技术开发区,6,7,8,Pros and Cons of Transgenics by Pronuclear Injection,Pros: Relatively simple and efficient Generate transgenics in 3 months Long transgenes possible Potentially all sp
4、eciesCons: Multiple copies at a single locus Occasionally mosaic founders (late stage integration) 10% insertions result in phenotype due to disruption ofendogenous gene Deletion, complex rearrangements of host genome Not all integrated transgenes are expressed. Expressionlevel do not correlate well with copy number,9,转基因平台建立,10,转基因平台运转模式,11,12,13,14,转基因平台迄今工作情况统计,15,将来发展,转基因小鼠制备速度加快，能够制备其它物种的转基因动物；建立制备基因敲除小鼠平台；建立ZFNs 、TALEN等新的靶向基因操作技术 ；,
