1、第九章生物反应工程习题解91、解DTDCCKCRRSSMSSMAX，02MAXECKR,因为35010010103SC，按拟一级处理，积分SMSCKRDTCMAXD，得SMSSXKTTKRCC11LNLNMAX0，代入T2，XS5，得K205011LN，所以K002565MIN1所以256501002565011LNSXXS1E025652262CSCS01XS3105102262232105MOL/LLMOLEECCKTSS/1039110353002565050MIN/105652MIN/1002565053MAXLMOLLMOLKKRM92、解/104LMOLCS410950520103
2、049001060051MIN/106LMOLRS177173125106806743/1013MOLLCS02441053192397092040894340196078MIN/1013MOLLRS56578800941251413233将米氏方程SMSSCKCRRMAX线性化MAXMAX11RCRKRSMS，以SR1对SC1作图，斜率为MAXRKM，截距为MAX1R由图知MAX1R761382，MAXRKM008094所以MAXR01313MIN/LMOL，MK0080901313001063LMOL/93解竞争性抑制米氏方程SIIMSRICKCKCRR1MAXSMRCKR,MAX非竞争性
3、抑制米氏方程1MAXMSIISRIKCKCCRR（SMAXCKRIM其中，MIIMMKKCKK1,MAX,MAXMAX,1RIIRIRKCRR线性化MAXMAXR11RCRKRSM竞争MMMAXMAX/11RCRKRSRMRI非竞争MMAXSAXMRIRCRKRIMIM11410SC/MOL/L反应初始速率/MOL/LMIN4101SCL/MOLRR1IR1无抑制有抑制LMOLCI/10224102817100357005881536230667002780043520432905002330034550655002001540020075746101330013500164分别以SC1对MA
4、X1R和SC1对IR1作图，求得两截距分别为001029和001012，截距基本相同所以为竞争抑制。斜率分别为00257和00498494解竞争抑制MAXMAX11RCRKRSMIRI（A）1IIMMIKCKKB（A）式变形为110MAXSMIRICKRR，又297MAX1021051103RIRRLMOLCRRKSIRMI/104102110213520MAX由B式，10111076104543IK解出12106751IKMOL/L95解OEHDCOONCCHBNHAOOHHC224080149070413252C2CDH63B1704C2EO12A0408C2DENB0149C660ADR
5、Q解方程组得A1222B01778C1193D0807E171745904612521461640801414907041121931/SXY5401043912521/ACYOX96解SSSRRCCVV00，SSSCKCDMAXMAXMAXMAX111SSSSSCKCCKD，以SC1对D1作图，斜率为AXSK，截距为MAX11/D/H16678334173232331591671521451411371/CS/M3/KG1667769303250156988265594548作图后与两轴的交点分别为1H和10M3/KG，所以31MAX/10,950MKGKHS、XXSXSMCRYR/1XSX
6、XXSXSSCMYDMCDCYDTDCR1/XSXSSCMYDCCD1/0，即SSXSXCCCMYDD0/1SISXISIXCCCDMY0/197、/104LMOLCS1301502002002002000MIN/LMOLRS124548606060/1014MOLLCS076920666705000005000005000005MIN/1MOLLRS008330022200208001670016700167将米氏方程SMSSCKCRRMAX线性化MAXMAX11RCRKRSMS，以SR1对SC1作图，斜率MAXRKM截距MAX1R当取第2、3、4三个点时经线性回归有斜率为00232截距为1
7、6104，所以RMAX625105MOLLMIN/4101SR4101SC当底物浓度远远大于MK时，SMSSCKCRRMAX可化简为MAXRRS，所以反应速率等于其最大反应速率为一常数。98、解SMSSCKCRRMAX，,/1077240LMOLCSLMOLKM/101313（1）MM方程的积分式为SMSSXRKRXCT11LNMAXMAX09011LN1058210131105829010772535411053MINV10L，其他条件不变，T不变，为11053MIN。T9011LN105821013110582901077253531975MIN99、解HMV/6030AMACRRCCCKCKCVVRCV0,0/AASXCCCYCAFMAFRAFMAFRCVKVCKVCKCKVV000300/5160502160MGVKVKVCRMAF910、解MAXMAX1111RCRKCKRSIIMRI111MAXMAXRIIIMSRIRRRKCKCR即1MAXSRIIIMRRICRKCKRR，竞争抑制IC低MAXSRIMRRICRKRR，B图1MAXSRIMIIRICRKKCRR，非竞争抑制IC低，MAXSRIMRRICRKRR，A图11MAXSRIIIMIIRICRKCKKCRR，反竞争抑制IC低，MAXSRIMRRICRKRR，C图
