微量残留病检测在急性白血病分层治疗中的意义,MRD:TheleukemicpopulationundectablebymorpglogicmethodshasbeendefinedasMRD,MinimalResidualDisease(MRD),MRDisatermusedwhenthereisevidence(immunophenotypic,molecular,orcytogenetic)thatleukemiccellsremainintheBMbutthereareinsufficientcellstobedetectedbyroutineexaminationunderthemicroscope.,MRD检测的方法,MRD监测的临床意义,MRD检测在分层治疗中的意义,FCM-MRD检测的结果,MRD检测方法,PCR:基因-定性、定量(RQ-PCR)b.多参数FCM:免疫标志,发病时寻找免疫标志和基因标志进行检测,目的基因:融合基因:BCR/ABL,AML1/ETO,PML/RAR基因重排:IgH,TCR(10-4-10-5)基因突变:FLT3-ITD,NPM1(10-