PB缓冲液.doc

.（四）0.2Mol/L PB缓冲液附表3 0.2mol/L PB缓冲液配制法pH0.2 Mol/L Na2HPO4（ml）0.2Mol/L NaH2PO4（ml）5.88.092.06.012.387.76.218.581.56.426.573.56.637.562.56.849.051.07.061.039.07.272.028.07.481.019.07.687.013.07.891.58.58.094.75.30.2Mol/L Na2HPO42 H2O溶液35.61g/LNa2HPO412 H2O溶液含71.64g/L0.2Mol/LNaH2PO4H2O溶液含27.60g/LNaH2PO42 H2O溶液含31.21g/L欲配0.1Mol/L PB缓冲液则在0.2Mol/L PB缓冲液的基础上加H2O稀释1倍即可。欲配制PBS液时，则在溶液中加0.85的NaCl溶液即可。