Topic #1: Bioengineering, the Amazing Red Blood CellsLab 4: Isolation of red blood cell ghost membranes.Date: Mon, 7/17/06Time: 1:35 3:35 pm Instructors: Weijuan Yao & Terrell GreenA) Objectives:Isolate the ghost membrane from mouse red blood cells.B) Materials Required:Mouse blood (200 l).Wash Buffer, pH 7.5 1L150 mM NaCl5 mM Sodium Phosphate:4 ml 1 M Sodium Phosphate Dibasic1 ml 1 M Sodium Phosphate Monobasic20 mg PMSF (Phenylmethylsulphonylfluoride)
