包涵体纯化方案缓冲液配方:1.变性复性缓冲液缓冲液A:50mM Tris-HCl (pH8.5), 1mM EDTA, 100mM NaCl,1%Triton X-100缓冲液B:50mM Tris-HCl (pH8.5), 1mM EDTA, 100mM NaCl,1%Triton X-100,2M脲素缓冲液C:50mM Tris-HCl (pH8.5), 1mM EDTA, 100mM NaCl,1%Triton X-100缓冲液D:50mM Tris-HCl (pH8.5), 1mM EDTA, 100mM NaCl,1%Triton X-100,2M盐酸胍溶解缓冲液E:50mM Tris-HCl (pH8.5), 1mM EDTA, 100mM NaCl,10mM -巯基乙醇/DTT,2mM脱氧胆酸钠,8M脲素复性缓冲液:50mM Tris-HCl (pH8.5),100mM NaCl ,6M/4M/2M脲素,1%甘氨酸,5%甘油,0.2%PEG,1mM氧化型谷胱甘肽,1mM还原型谷胱甘肽2.纯化缓冲液Bi