叶绿体色素含量的测定 -分光光度法中文摘要：本实验利用菠菜叶作为原料用80%的丙酮提取叶绿体色素，应用分光光度计在某一特定波长下所测定的吸光度，带入公式计算出色素溶液中各色素浓度。英文摘要：In this experiment , we used spinach leaves as raw material and used 80% of acetone to extract pigments from them ,and apply spectrophotometer at a particular wavelength to determine absorbance, then take the absorbance into the formula to calculate concentration of each pigment.实验原理：利用80%丙酮提取叶绿体色素，叶绿素a、叶绿素b和类胡萝卜素最大吸收峰的波长分别是663nm、646nm和470nm。根据分光光度计测定的吸光度值，可以计算出丙酮提取液中叶绿体色素含量。实
