各种pH值的Tris缓冲液的配制(共3页).doc

精选优质文档-倾情为你奉上各种pH值的Tris缓冲液的配制所需PH值（25）0.1mol/L HCI体积7.1045.77.2044.77.3043.47.4042.07.5040.37.6038.57.7036.67.8034.57.9032.08.0029.28.1026.28.2022.98.3019.98.4017.28.5014.78.6012.48.7010.38.808.58.907.0某一特定pH的0.05mol/L Tris缓冲液的配制：将50ml 0.1mol/L Tris碱溶液与上表所示相应体积（单位：ml）的0.1mol/L HCl混合，加水将体积调至100ml。Tris-buffered saline (TBS) (25 mM Tris) (1 liter)1. Weigh out 8 g NaCl, 0.2 g KCl and 3 g Tris base. 2.