细胞形态学检查 n 一、倒置显微镜观察细胞的形态 n (一)一般的形态学观察: n (二)用相差显微镜观察细胞的形态 n (三)荧光显微镜观察细胞的形态和结构n Identification of cultured adult rat RGCs. Cultured cells were co-labeled with anti-Thy-1 antibody (A), anti-NF-L antibody (B), and DAPI (C). (D) A digitally merged image simultaneously represents all three fluorescent labels. (E) The corresponding phase-contrast image. 免疫荧光染色法 n 用于标记二抗体的荧光素: n (1)异硫氰酸荧光素(FITC) n 发射的荧光波长为490619(绿色荧光) n (2)四乙基罗丹明:荧光波长为540660 (橙红色荧光) n (3)DAPI或Hoechst33258 : 染核,用紫外 激发 n 免疫荧光的染色步骤 n 1、