1、第5章细菌感染的检查方法Laboratory DiagnosisOutline Laboratory diagnosis for bacterial infections Morphological - microscopic Molecular Serological Laboratory diagnosis for viral infections Morphological - electron microscopic Isolation Molecular: nucleic acids and proteins Serological Laboratory diagnosis for
2、fungal infections Morphological - microscopic IsolationAssays Morphological assays Light or electron microscopies Isolation and differentiation Serological assays Antigen-antibody assays Molecular assays Microorganisms gene (DNA & RNA)Principles for Specimen Collection Sterile manipulation, avoid co
3、ntamination Obtain a specimen from the infected site according to the disease phase before administrating antibiotics Double sera for antibody detections, acute and recovery phase each Positive if the titer of the later specimen is above 4X to the first specimen Transport, and store correctly as soo
4、n as possible Store at -4oC for most bacterial specimens, but keep warm for Neisseria meningitidis and Neisseria gonorrhoeae Store at -4oC -70oC for viral specimensLaboratory Diagnosis for Bacterial Infections Morphological Protein and Nucleic acids AntibodyBacteriological Diagnosis Identifying the
5、organismMorphological Isolation & cultureBiochemical reaction Serological assays Pathogenesis & antibiotics susceptibilityAnimal experimentVirulence testantibiotics susceptibility Diagnosis Strategy for Bacterial InfectionMorphological Non-stained microscopic observationDark-field microscopyObserving the movement of live bacteria Stained microscopic observationsGram stainAcid-fast stainFluorescence stain
